Signalling

Part:BBa_K395164:Design

Designed by: Kaneta Yusuke   Group: iGEM10_Tokyo_Tech   (2010-10-05)


KanR activated by lasR and 3OC12HSL(AHL of Las family)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to create Kanamycin resistance gene on pSB6A1activated by LuxR and 3OC12HSL.As a vector,We already create Kanamycin resistance gene on pSB6A1.To accomplish it, vector was cutted by EcoRI and Xha while insert(promotor) was cutted by EcoRI and SpeI



Source

References